Validation of a Multiplex PCR for the Simultaneous Detection of E. coli and Class 1 Integron

Antibiotic resistant E. coli in the animal gut can promote transfer of antibiotic resistance between and human. In order to enforce National Action Plan Bangladesh, it is imperative detect presence harbouring Class 1 integrons that potentially genes among bacteria. Previously used primers pairs were a multiplex PCR successfully species-specific amplicon integron 57% isolates. Plasmid was absent profiling indicated highest oxytetracycline (89%) lowest streptomycin (18.52%). A total 38% isolates found be against 8 10 antibiotics used, 85.19% being multidrug resistant. However, pattern could not specifically correlated integron. The validated as quick method simultaneously. Plant Tissue Cult. & Biotech. 33(1): 17-24, 2023 (June)